Rhodamine is a family of related chemical compounds, fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are used as a dye and as a dye laser gain medium.[1][2] They are often used as a tracer dye within water to determine the rate and direction of flow, transport and pathway detection. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Liquid dyes can used to qualitatively calibrate the fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. They are soluble in water, methanol and ethanol.
Rhodamine dye product is not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment.

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