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EXO Total Algae sensor

The EXO total algae sensor is a dual-channel fluorescence sensor that generates two independent data sets; one resulting from a blue excitation beam that directly excites the chlorophyll a molecule, present in all photosynthetic cells, and a second from an orange excitation beam that excites the phycocyanin accessory pigment found in blue-green algae (cyanobacteria). This orange excitation triggers a transfer of energy from the phycocyanin to the central chlorophyll a, where photosynthesis is initiated. Although blue-green algae contain chlorophyll a, the chlorophyll fluorescence signal detected by in situ fluorometers is weaker than in eukaryotic phytoplankton. This results in an underestimate of algae biomass when using a single-channel chlorophyll sensor when blue-green algae are present. The EXO total algae sensor generates a more accurate total biomass estimate of the planktonic autotrophic community by exciting chlorophyll a and phycocyanin.

BGA probes and Chlorophyll probes have been combined into a single sensor

A change with the EXO platform will be the BGA probes and Chlorophyll probes have been combined into a single sensor. There is no option to purchase one or the other.

This sensor is referred to as the “Total Algae” Sensor because having both chlorophyll and algae are important for estimating the total algae bio mass; the sensor will still output a BGA reading and a Chlorophyll reading independently.

SPECIFICATIONS

Blue-green Algae Phycocyanin
Range: 0 to 100 μg/L; 0 to 100 RFU;
Linearity: R2 >0.999 for serial dilution of Rhodamine WT solution from 0 to 100 μg/mL BGA-PC equivalents
Detection Limit: 0.04 μg/L PC
Response: T63<2 sec
Resolution: 0.01 μg/L; 0.01 RFU

Chlorophyll
Range: 0 to 400 μg/L Chl; 0 to 100 RFU
Linearity: R2 >0.999 for serial dilution of Rhodamine WT solution from 0 to 400 μg/L Chl a equivalents
Detection Limit: 0.09 μg/L Chl
Response: T63<2 sec
Resolution: 0.01 μg/L Chl; 0.01 RFU

Ordering Information:
599102-01 EXO Total Algae - PC Sensor, Ti "EXO Total Algae - PC Sensor, Ti
- Optimised for freshwater use - Phycocyanin
- Includes chlorophyll and blue green algae sensors in a single Sensor
- Incorporates wet-mate connector and sealed welded titanium design"

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